HLJDT induced apoptosis in U266 cells. (A) U266 cells were cultured with 0, 25, 50, 100, or 200 ng/mL of HLJDT (•), GZFLW (♦), or HLT (▴) for 24 hours and then the cells were applied to a flow cytometer. Viable and nonviable cells were assessed by FS/SS. Values shown in this figure are the mean ± 1 SD of 3 independent experiments. ^*P < .05, ^**P < .01. (B) The mitochondrial membrane potential of U266 cells was assessed after treatment with 0, 50, 100, or 200 ng/mL of HLJDT, GZFLW, or HLT for 12 hours. Values shown in this figure are the mean ± 1SD of 3 independent experiments on flow cytometric detection of DiOC₆ uptake at indicated time points. ^*P < .05, ^**P < .01. (C) Lysates from U266 cells treated with 50, 100, or 200 ng/mL of HLJDT for 12 hours were assessed for cleaved caspase by Western blotting with antibodies to caspase-9 and -3.