Figure 4.
Identification of baicalein as the active component ofScutellariaradix (root) in HLJDT. (A) U266 cells were cultured with HLJDT (▴), HLJDH without Scutellaria root (♦), or Scutellaria root alone (•) at the indicated concentration for 4 days. Viable cell number was assessed by flow cytometric detection of FS/SS. Values are the mean ± 1 SD of 3 independent experiments. *P < .05, **P < .01. U266 (B), ILKM2 (D), and AMO1 (E) cells were cultured with baicalein (•), baicalin (▴), or wogonin (♦) at the indicated concentration for 4 days. Viable cell number was assessed by flow cytometric detection of FS/SS. Values are the mean ± 1 SD of 3 independent experiments. *P < .05, **P < .01. (C) U266 cells were also cultured with baicalein (▪), baicalin (▦), or wogonin (□) at the indicated concentration for 4 days. Nonviable cells were quantitated by flow cytometric detection of FS/SS. Values are the mean ± 1 SD of 3 independent experiments. *P < .05, **P < .01.