American Society of Hematology

Figure 5.

$Figure 5. Flow cytometric histogram of cells cultured with baicalein. Bone marrow mononuclear cells from a representative myeloma case were cultured with DMSO control or baicalein (1.0, 12.5, or 25.0 μM) in the presence of 5 ng/mL of IL-6 for 2 days. After the culture, the cells were harvested, stained with FITC-anti-CD38, PE-anti-MPC-1, and PC5-anti-CD45 antibodies and applied to a flow cytometer. Viable cell numbers of CD38++, CD38++MPC-1-, and CD38++MPC-1+ fractions were examined, and values shown in this figure are the number detected for a given period (1 minute analysis) for total cells (A), CD38++MPC-1- cells (F), and CD38++MPC-1+ cells (G), respectively.$

Flow cytometric histogram of cells cultured with baicalein. Bone marrow mononuclear cells from a representative myeloma case were cultured with DMSO control or baicalein (1.0, 12.5, or 25.0 μM) in the presence of 5 ng/mL of IL-6 for 2 days. After the culture, the cells were harvested, stained with FITC-anti-CD38, PE-anti-MPC-1, and PC5-anti-CD45 antibodies and applied to a flow cytometer. Viable cell numbers of CD38⁺⁺, CD38⁺⁺MPC-1^-, and CD38⁺⁺MPC-1⁺ fractions were examined, and values shown in this figure are the number detected for a given period (1 minute analysis) for total cells (A), CD38⁺⁺MPC-1^- cells (F), and CD38⁺⁺MPC-1⁺ cells (G), respectively.

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