American Society of Hematology

Figure 3.

$Figure 3. Plastic-immobilized antigen induces full activation of unprimed T cells. (A) Purified splenic T cells were cultured for 3 days in plates coated with either FγG, FγG-TNP, anti-CD3, or both anti-CD3 and anti-CD28 mAbs. As in Figure 2, cells were costained for Thy1.2 and CD25 expression to evaluate the IL-2Rα expression on T cells as a measure for activation. □ represents WT; ▦, Tg 8.4; and ▪, Tg 8.7. (B) Kinetics of IL-2 production in supernatants of purified splenic T cells, stimulated as described. (C) Proliferation of the same cultures assessed by the XTT assay. Results represent 4 separate experiments with 3 mice/group. (B-C) □ indicates FγG; ▴, FγG-TNP; ○, anti-CD3;.and •, both anti-CD3 and anti-CD28 mAbs. The percentage in the dot plot represents the fraction of lymphocytes that score positive for both Thy1.2 and CD25+. The error bars represent the standard deviation of triplicates.$

Plastic-immobilized antigen induces full activation of unprimed T cells. (A) Purified splenic T cells were cultured for 3 days in plates coated with either FγG, FγG-TNP, anti-CD3, or both anti-CD3 and anti-CD28 mAbs. As in Figure 2, cells were costained for Thy1.2 and CD25 expression to evaluate the IL-2Rα expression on T cells as a measure for activation. □ represents WT; ▦, Tg 8.4; and ▪, Tg 8.7. (B) Kinetics of IL-2 production in supernatants of purified splenic T cells, stimulated as described. (C) Proliferation of the same cultures assessed by the XTT assay. Results represent 4 separate experiments with 3 mice/group. (B-C) □ indicates FγG; ▴, FγG-TNP; ○, anti-CD3;.and •, both anti-CD3 and anti-CD28 mAbs. The percentage in the dot plot represents the fraction of lymphocytes that score positive for both Thy1.2 and CD25⁺. The error bars represent the standard deviation of triplicates.

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