Cytolytic activity mediated by TPCR-redirected transgenic cells. Cytotoxicity of purified T cells from Tg8.7 and WT mice as effectors (E) was assayed using TNP-modified A20 cells as targets (T) at the indicated E/T ratios. Lysis was determined after incubation for 6 hours at 37°C using the LDH kit as described in “Materials and methods.” ♦ indicates A20 plus Tg 8.7 cells; ▪, A20-TNP plus Tg 8.7; ○, A20 plus WT; and ▵, A20-TNP plus WT. Error bars represent the standard deviation of replicates.