HC IgEs and IgE+Ag induce the migration of BMMCs through FcϵRI and β1 integrin. (A) HC, but not PC, IgEs induce BMMC migration efficiently in an 8-hour transwell assay. BMMCs, sensitized overnight with anti-DNP IgE (206) and washed, were also attracted to a lower well containing DNP-HSA. For comparison, SCF (100 ng/ml) was also tested in the same assay. Also shown are dose-response (B) and time-course (C) experiments using 10 μg/mL 206 IgE, 10 μg/mL SPE-7 IgE, 10 ng/mL DNP-HAS, and 100 ng/mL SCF. (D) Migration assays were performed with FcϵRIα^-/- and control BMMCs. (E) Migration of wild-type BMMCs was inhibited by neutralizing antibodies against integrins. Experiments were performed in triplicate. Representative data (± SD) are shown out of 2 or more experiments.