Comparison between HC IgE- and chemokine-induced migration of BMMCs and checkerboard analysis of HC IgE-induced mast cell migration. (A) SPE-7 IgE-induced migration of BMMCs was compared with those induced by mast cell-attracting chemokines, MIP-1α, MCP-1, and RANTES. (B-C) BMMC migration was induced by SPE-7 IgE in combination with chemokines or SCF. (D) BMMCs in upper wells containing various concentrations of SPE-7 IgE were incubated with various concentrations of SPE-7 IgE in lower wells. (E) BMMCs in upper wells containing various concentrations of SCF were incubated with various concentrations of SCF in lower wells. Values (mean ± SD) are shown out of 2 independent experiments.