![Figure 4. Involvement of adenosine, LTB4, and chemokines in an autocrine/paracrine mechanism of IgE- and IgE+Ag-induced mast cell migration. (A) CFSE-labeled wild-type and FcϵRIα-/- BMMCs in upper wells were attracted to lower wells containing wild-type cells and SPE-7 or 206 IgE-sensitized wild-type cells and antigen. (B) Supernatants of wild-type BMMCs incubated with SPE-7 for the indicated periods attracted FcϵRIα-/- cells. Supernatants of PC IgE (206)-sensitized wild-type cells incubated with antigen for the indicated periods also attracted FcϵRIα-/- cells. (C) Migration was induced by adenosine and LTB4, but not by sphingosine 1-phosphate (S1P) or histamine. (D) Pertussis toxin (PTX), adenosine deaminase (ADA), and adenosine A3 receptor inhibitor MRS 1523 inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen or adenosine. (E) 5-Lipoxygenase inhibitor (N-oleoyl dopamine [ODA]), BLT1 receptor inhibitor (U-75302), and BLT2 inhibitor (LY2552833) inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen. U-75302 and LY2552833 at 1 μM each were used in a combination (U+LY) as well. (F) Neutralizing antibodies to several chemokines inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen. All the antibodies at the higher concentrations each were used in a combination (all) as well. Mean values ± SD are shown out of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/8/10.1182_blood-2004-11-4205/6/zh80080577320004.jpeg?Expires=1735504256&Signature=Xjkmaabv6X4FVprdsHlYobEbByqfUTNl7-uydxf0SJFx3cNV83-g0UnsQ0cYZ0Oo4w30~HvHrGfbZtqOCPUdBwqQfYbgdRW2Mf52pqtptwfUw7V~Itd0OIM-LJpSa58c8PWFGJFSA-RBRo5J2Al~WjxtYQozpwL3-yi5Axkzgm7IZQ1WZFIm6L7hZUKrHSxVmjFvlUQgiXQtxcs88lnk8JeyE-g6xtGf3veyR9kBWFIJi1oGW9QMrGlfDbSlJ9hlReJcfvc2~Qc8p2lisXZxPy4HLdtmWw7VpiOvyc76V2Fpwp0owPBQH~k7sX-jWe9CEOnmUPJG8zgDvYROwW62ZQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Involvement of adenosine, LTB4, and chemokines in an autocrine/paracrine mechanism of IgE- and IgE+Ag-induced mast cell migration. (A) CFSE-labeled wild-type and FcϵRIα-/- BMMCs in upper wells were attracted to lower wells containing wild-type cells and SPE-7 or 206 IgE-sensitized wild-type cells and antigen. (B) Supernatants of wild-type BMMCs incubated with SPE-7 for the indicated periods attracted FcϵRIα-/- cells. Supernatants of PC IgE (206)-sensitized wild-type cells incubated with antigen for the indicated periods also attracted FcϵRIα-/- cells. (C) Migration was induced by adenosine and LTB4, but not by sphingosine 1-phosphate (S1P) or histamine. (D) Pertussis toxin (PTX), adenosine deaminase (ADA), and adenosine A3 receptor inhibitor MRS 1523 inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen or adenosine. (E) 5-Lipoxygenase inhibitor (N-oleoyl dopamine [ODA]), BLT1 receptor inhibitor (U-75302), and BLT2 inhibitor (LY2552833) inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen. U-75302 and LY2552833 at 1 μM each were used in a combination (U+LY) as well. (F) Neutralizing antibodies to several chemokines inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen. All the antibodies at the higher concentrations each were used in a combination (all) as well. Mean values ± SD are shown out of 2 independent experiments.
