Aberrant subcellular localization of NE in G185R cells. (A) Confocal immunofluorescence microscopy shows the subcellular distribution of NE (green) with counterstaining of nuclei (Hoechst, blue). (B) Immunoblot analysis of subcellular fractions from WT NE- and G185R NE-transduced cells blotted with an antibody to NE. NE is seen as a broad low molecular-weight species localizing to fractions 4 to 6 in G185R cells. (C) The blot in panel B was stripped and reblotted with antibody to MPO showing an identical distribution pattern for MPO in WT- and G185R-transduced cells.