Figure 3.
Survival curve and lung histopathology from CLP-operated mice challenged with A fumigatus alone or coinjected with BMDCs. (A) At day 5 after CLP surgery, groups of mice were challenged intratracheally with either 5 × 107 conidia alone or coinjected with BMDCs, and mouse survival was analyzed up to 7 days after A fumigatus challenge. Each group contained 5 mice. P < .05 between CLP + Asp and CLP + Asp + BDMC. (B) At day 15 after surgery, all mice were challenged via intratracheal injection with 5 × 107 conidia alone (i) or together with 1 × 106 BMDCs obtained from 15-day sham-operated mice (ii) or obtained from 15-day CLP mice (iii). The lungs were collected at day 10 after challenge and processed for histology. Paraffin-embedded lung sections were stained with GMS (fungal material appears black in lung sections [black dots]). Original magnification was × 20 for all panels.