Mutations in CTAR-1 and CTAR-2 abrogate the inhibitory effect of LMP-1 on BAX promoter activity. (A) Schematic presentation of LMP-1 and LMP-1-activated signaling pathways. Alterations in mutated LMP-1 proteins are indicated (PQT > AAA, Y384G, ΔCOOH). (B) HEK 293-T cells were cotransfected with plasmids encoding wild-type LMP-1 or various mutants of LMP-1 (see Figure 2A) and with pBAX-CAT. The expression of wild-type LMP-1 and the respective mutants was analyzed by Western blot. Detection of GAPDH was included as loading control. (C) Quantitative evaluation of CAT assay of HEK 293-T cells transfected with pBAX-CAT (left side) or pSV2-CAT (right side) and wild-type or mutant pLMP-1. Mean values and standard deviations of 3 independent experiments are shown; ^*P < .05.