Jagged2 induces the development of NK cells from Lin^- Sca-1⁺ c-Kit⁺ HSCs. Lin^- Sca-1⁺ c-Kit⁺ HSCs were cocultured with OP-9S/LXSN (negative control) or OP-9S/LMJSN (“J2”) in the presence of IL-7 and Flt3L for 19 days without or with IL-2 on days 10 to 19 and analyzed for the expression of NK1.1 versus CD3, CD19 versus B220, and CD8 versus CD4. The percent of CD3^-NK1.1⁺ cells (left upper quadrant) is indicated.