Figure 5.
Figure 5. Localization of Rap1GAP2 in transfected COS-1 cells and in platelets. COS-1 cells transiently transfected with Rap1GAP2 (A) and nontransfected control cells (B) were stained with an antibody specific for Rap1GAP2. Rap1GAP2 localized predominantly to cytosolic and perinuclear structures in transfected cells. Nontransfected control cells were not labeled, indicating specificity of the antibody. (C-H) Human platelets spread on glass were double labeled with antibodies specific for Rap1GAP2 (C, F) and Rap1 (D, G, and merged images E, H). (F-H) In the center of each panel, a single large, spread platelet is shown. Both Rap1GAP2 and Rap1 proteins partially colocalized in central vesicular structures. Shown are examples of experiments performed at least 5 times. Bar = 10 μM.

Localization of Rap1GAP2 in transfected COS-1 cells and in platelets. COS-1 cells transiently transfected with Rap1GAP2 (A) and nontransfected control cells (B) were stained with an antibody specific for Rap1GAP2. Rap1GAP2 localized predominantly to cytosolic and perinuclear structures in transfected cells. Nontransfected control cells were not labeled, indicating specificity of the antibody. (C-H) Human platelets spread on glass were double labeled with antibodies specific for Rap1GAP2 (C, F) and Rap1 (D, G, and merged images E, H). (F-H) In the center of each panel, a single large, spread platelet is shown. Both Rap1GAP2 and Rap1 proteins partially colocalized in central vesicular structures. Shown are examples of experiments performed at least 5 times. Bar = 10 μM.

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