Transduction of murine bone marrow cells and ectopic Shp-2 expression. (A) Low-density bone marrow mononuclear cells from C57/Bl6 mice were transduced with ecotropic retroviral supernatants (pMIEG3, pMIEG3-WT, pMIEG3-E76K, pMIEG3-D61V, and pMIEG3-D61Y) and tranduced cells were enriched by sorting for EGFP expression. Representative FACS showing the percentage of EGFP⁺ cells for collection of transduced cells is demonstrated. (B, left) Western blot analysis demonstrated that the exogenously introduced Shp-2 proteins are equally expressed in each group of transduced cells. Shp-2 band intensities normalized to GAPDH are shown graphically (right). Representative Western blot of 2 independent experiments with similar results.