EGFP-bcl-2 alters T-cell development when expressed in thymocytes. FACS analysis of GFP-positive thymus cell populations from 10-week-old rag2-GFP and rag2-EGFP-bcl-2 transgenic zebrafish. Gated populations of erythrocytes (red), lymphocytes (blue), granulocytes and monocytes (green), and blood cell precursors (purple) are outlined in color. Populations of cells within each gate are described as percentages of total live cells (1 SD; n = 6). Total GFP-positive populations (left column) and sorted, reanalyzed cell populations gated (middle and right columns). Cell size is represented by forward scatter (FSC), and granularity by side scatter (SSC).