Cell surface expression of wild-type G-CSF-R and G-CSF-R_785Lys. Flow cytometric analysis of G-CSF-R expression on Gcsfr^-/- bone marrow cells retrovirally transduced with wild-type G-CSF-R or G-CSF-R_785Lys revealed no differences in transduction efficiency (8.02% versus 7.35%) and receptor surface expression (mean fluorescence intensity, 246.8 versus 231.4). The second row of dot plots and thin line in histograms indicate cells stained with biotinylated G-CSF-R antibodies and SA-PE; the first row and bold line in histograms indicate cells stained with SA-PE only.