TLR4 and MD2 mRNA transcripts are up-regulated in purified primary human monocytes by SEB. Quantities of TLR4 (A) and MD2 (B) mRNA were corrected relative to the constitutively produced β-actin gene product. Individual results are shown for SEB-stimulated (⬡) or TCM-only exposed (○) monocytes from 5 donors at 3 hours, 7 hours, and 24 hours. The difference in TLR4 and MD2 expression in SEB- and TCM-stimulated cells was significant (repeated measures analysis of variance, P < .05). The result was representative of 2 independent experiments.