American Society of Hematology

Figure 3.

$Figure 3. PPF in BACH1 transgenic mice and their wild-type littermates. The megakaryocyte fractions (enriched by a BSA density gradient) were incubated in IMDM supplemented with 1 × Nutridoma-SP. After 20 hours of incubation, acetylcholine esterase activity was detected before counting the megakaryocytes to determine their PPF. The number of acetylcholine esterase-positive cells displaying the obvious filamentous cell projections was counted and the PPF ratio (number of megakaryocytes with PPF–total megakaryocytes) was calculated. (Left) The mean ± SD of PPF ratios from 3 independent experiments are shown. Typical proplatelet-producing (middle) and -nonproducing (right) megakaryocytes are shown. Cell images were obtained using an Olympus IX71 inverted microscope equipped with an LCPlan FI 40 ×/0.60 objective lens and an Olympus SC35 camera (Olympus).$

PPF in BACH1 transgenic mice and their wild-type littermates. The megakaryocyte fractions (enriched by a BSA density gradient) were incubated in IMDM supplemented with 1 × Nutridoma-SP. After 20 hours of incubation, acetylcholine esterase activity was detected before counting the megakaryocytes to determine their PPF. The number of acetylcholine esterase-positive cells displaying the obvious filamentous cell projections was counted and the PPF ratio (number of megakaryocytes with PPF–total megakaryocytes) was calculated. (Left) The mean ± SD of PPF ratios from 3 independent experiments are shown. Typical proplatelet-producing (middle) and -nonproducing (right) megakaryocytes are shown. Cell images were obtained using an Olympus IX71 inverted microscope equipped with an LCPlan FI 40 ×/0.60 objective lens and an Olympus SC35 camera (Olympus).

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