Figure 4.
DNA contents of CD41+ bone marrow cells in BACH1 transgenic mice and their wild-type littermates. Unfractionated bone marrow cells were labeled by FITC-conjugated anti-CD41 antibody and DNA content was stained with propidium iodide. Two-color flow cytometry was used to detect the DNA content in megakaryocytes. A typical histogram showing DNA content averaged over 3 experiments is shown. The transgenic lines and the number of CD41+ cells among 2 × 106 nucleated cells that were analyzed are indicated inside each graph.