Semiquantitative RT-PCR analysis of p45 target genes in megakaryocytes derived from BACH1 transgenic mice. Expression of β1-tubulin, β5-tubulin, TXAS, Hsd3b, GATA-1, p45, and Hprt was analyzed by RT-PCR using RNA extracted from cultured megakaryocytes derived from fetal liver cells of wild-type mice and their transgenic littermates. To ensure linearity of PCRs, 10-fold serial dilutions of template cDNA were used. Typical photographs from 3 independent experiments are shown.