Time course of bid expression after CD40 ligation on CLL B cells. In panel A the CLL cells were cocultured 1 day with HeLa cells or HeLa-CD154 that expressed the CD40 ligand. The CLL cells were separated from the adherent HeLa cells, cultured separately, and then used to prepare whole-cell lysates. Forty micrograms of cell lysate was applied to each lane of a polyacrylamide gel for immunoblot analyses with anti-Bid (top row) or anti–β-actin (bottom row), as indicated on the right side of the figure. The different lanes are of lysates prepared from CLL cells cultured for 1 day after coculture with HeLa cells (lane 1) or for 1 day (lane 2), 2 days (lane 3), or 4 days (lane 4) after coculture with HeLa-CD154 cells. In panel B, CLL cells were isolated from a patient before (lane 1), 1 day after (lane 2), or 14 days after (lane 3) infusion of 1 × 109 Ad-CD154–transduced autologous leukemia cells, as described.1 Forty micorgrams of cell lysate was applied to each lane for immunoblot analysis, as described in panel A.