Figure 2.
Hemin induction of K562 cells. (A) A DNA SATB1 probe ( \(\mathrm{Wt}_{7}^{25}\)
)17 used for EMSA shows a high-molecular-weight band (SATB) from K562 extract. The radiolabeled probe was incubated with no extract (ne, lane 1) or K562 extract before and after hemin induction, indicated in hours (lanes 2-6). Lane 7 contains uninduced K562 cell extract and cold probe as competitor. The large faster-migrating band in lanes 5 to 7 represents nonspecific binding. (B) Western blotting using anti-SATB1 serum and 5 μg protein of K562 cell extract following hemin induction indicated in hours (lanes 2-5) shows the 96-kDa band up to 48 hours after hemin induction. As loading control, β-actin (βac) is also indicated (lanes 6-9). Lane 1 contains molecular weight markers. (C) The γ- and ϵ-globin mRNAs from K562 cells were quantified before and after hemin induction (indicated in hours). Results were normalized to the level of β-actin mRNA. Error bars represent SD from 3 independent measurements.
![Figure 2. Hemin induction of K562 cells. (A) A DNA SATB1 probe (\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{Wt}_{7}^{25}\) \end{document})17 used for EMSA shows a high-molecular-weight band (SATB) from K562 extract. The radiolabeled probe was incubated with no extract (ne, lane 1) or K562 extract before and after hemin induction, indicated in hours (lanes 2-6). Lane 7 contains uninduced K562 cell extract and cold probe as competitor. The large faster-migrating band in lanes 5 to 7 represents nonspecific binding. (B) Western blotting using anti-SATB1 serum and 5 μg protein of K562 cell extract following hemin induction indicated in hours (lanes 2-5) shows the 96-kDa band up to 48 hours after hemin induction. As loading control, β-actin (βac) is also indicated (lanes 6-9). Lane 1 contains molecular weight markers. (C) The γ- and ϵ-globin mRNAs from K562 cells were quantified before and after hemin induction (indicated in hours). Results were normalized to the level of β-actin mRNA. Error bars represent SD from 3 independent measurements.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/8/10.1182_blood-2004-08-2988/6/zh80080577250002.jpeg?Expires=1735534179&Signature=AdjtJ4UQR62KbANEshqXp~jjivyxbnFqzG5G2zi~7k0x77SQOg-0nnOr6sm6Gn99q~urGxUlT1dQPj~szrHy7~tLIUJZh~r5AU-bb5~NXKauakRqsV5Kw0F2r4L89aIIkUiHzzPGyMMot-LEv8v-1AsiW6zd1hxjsLfEa6pKTBSCm45YIJOJEU9Jzbv2CK5drM7MDuV6pIV-nhvyeApW41PcwpBa5EJSi4IvrG8oA9vxt0XFjimI96uO1HuQq2COOhSzENh3WVbOfxCaamOhwNrhBS4MoYaRai2~FfiqDTtmLuBQzbirNBmrpoKhd4yFWjQF59DjyNpYfUb67nqiIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
