Histone modifications by SATB1 in K562/SATB1 cells. (A) ChIP DNA isolated using specific antiacetylated and antimethylated histone antibodies was subjected to quantitative real-time PCR analysis using specific primer pairs and TaqMan probes for HS2 and the ϵ-, γ-, and β-globin promoters as indicated. (B) ChIP DNA isolated with antibodies specific for acetylated histone H3 (H3) and acetylated histone H4 (H4), and histone H3 methylated at lysine 9 (H3-MeK9) from K562 (K; □) and K562/SATB1 (S; ▪) cells were analyzed. (C) ChIP analysis was repeated following hemin induction at 0 (□), 24 (▦), and 48 hours (▪) of K562 cells. Error bars represent SD from 3 independent experiments.