Allostimulatory function of DCs pulsed with HCV-LPs. (A) Responder PBMCs (2 × 10⁵ cells/well) were cultured for 6 days in the presence of increasing concentrations of irradiated allogenic stimulator DCs pulsed with HCV-LPs (⬡), insect cell control preparation (▪), or without antigen (▵). T-cell proliferation was measured by [³H]thymidine incorporation during the last 16 hours of culture. The results are expressed as counts per minute (cpm; mean ± SD of an experiment performed in triplicate). (B) IL-4/IFN-γ production by allogeneic CD4+ T cells. CD4+ T cells were cocultured with DCs (T/DCs ratio: 10:1) as described for panel A. For IL-4 and IFN-γ determination, cell culture supernatants were collected on days 1 and 5, respectively. Data are shown as pg/mL (mean ± SD of an experiment performed in triplicate). The thresholds for cytokine detection are indicated as - - -.