Perforin expression by Western blot analysis. Analysis is shown under reducing (A) or nonreducing (B) conditions in FHL patients. T cells from healthy control (C), FHL2 patients, and FHL3 patients were grown as described in “Generation of alloantigen-specific CTL lines and analysis of CTL cytotoxicity.” Cell extracts of these cells (20 μg each) were analyzed by Western blot with antiperforin antibody. The data shown are representative results of 3 independent experiments. NS indicates nonsense mutation; and MS, missense mutation. The numbers above the lanes correspond to the UPNs of patients listed in Table 2.