Wnt3a enhances proliferation of 32D/Flt3 cells. The 32D/Flt3-WT (A) and 32D/Flt3-ITD (B) cells were starved from growth factors for 12 hours and subsequently exposed to a combination of FL or IL-3 and Wnt3a-conditioned medium (CM; ▪) or a control-conditioned medium (□) for 6 hours. DNA synthesis was analyzed in triplicate by ³H-thymidine incorporation over 12 hours. Each bar corresponds to the mean incorporated radioactivity ± SD. The significance of differences between Wnt3a CM and control CM was calculated according to the Student t test.