Influence of Flt3-ITD on Wnt signaling pathways. 32D cells stably transfected with Flt3-WT or with Flt3-ITD were grown for 12 hours in the absence of IL-3. Subsequently, cells were stimulated with FL, IL-3, or left without cytokine for 6 hours. Additionally, either 50% of Wnt3a-conditioned medium or control-conditioned medium was added. Lysates were subjected to Western blot analyses with the indicated primary antibodies.