SiRNA knocks down Fas in Jurkat cells and reduces their susceptibility to Fas-mediated death. Jurkat cells were transduced with the EV (A), CsiRNA (B), siRNA8 (C), siRNA10 (D), or sequentially with siRNA10 and GFP-siRNA8 (siRNA10 and 8) (E). After selection, cells were stained with anti-CD95-PE (solid line) or isotype control (dashed lines). (F) The RT-PCR is shown for Fas (top) and β-actin (bottom) using the total RNA extracted from Jurkat cells. (G) Fas mediated-apoptosis is shown in Jurkat cells incubated with (▦) or without (□) CH-11 antibody (20 ng/mL). Data represent the mean ± SD of 3 independent experiments (^*P = .01; ^**P = .001). (H) The PARP protein cleavage in Jurkat cells is shown after incubation with or without CH-11 antibody (top gel). The bottom gel shows the membrane reprobed with anti-β-actin antibody.