SR-bFGF-maintained hESCs undertake hematopoietic development. In comparison with MEF-CM-cultured hESCs (left column), SR-bFGF-cultured hESCs (right column) displayed the similar following properties after differentiation: (A) formation of hEBs (bar = 100 μm), (B) generation of CD45^negPFV hemogenic precursors at day 10 of hEB development,⁹  (C) expression of the panleukocyte hematopoietic marker CD45 and primitive hematopoietic marker CD34 at day 22 of hEB development, and (D) generation of hematopoietic progenitors determined by clonogenic colony-forming unit assay at day 22 of hEB development.