Hematopoietic cells derived from SR-bFGF-maintained hESCs have characteristic hematopoietic progenitor properties. Hematopoietic cells derived from SR-bFGF-maintained hESCs were identical to those derived from MEF-CM-maintained hESCs and produced characteristic hematopoietic CFU subtypes, including erythroid (A), macrophage (B), granulocyte (C), and granulocyte-macrophage colonies (D); scale bars = 100 μm. The cells prepared from an erythroid colony showed mature enucleated erythrocytes (arrowheads) and immature erythrocytes (E), from a macrophage colony showed macrophage morphology (F), and from a granulocyte colony showed mature neutrophils and eosinophils (G); Wright Giemsa staining; scale bars = 10 μm. The cells from erythroid colonies were stained positive for erythroid marker glycophorin A and negative for panleukocyte marker CD45 and myelomonocytic markers CD33 and CD13 (H-I). In contrast, the cells from granulocyte colonies were stained negative for glycophorin A and positive for CD45, CD33, and CD13 (J,K).