Changes in cutaneous lymphatic vessels after adenoviral transduction. (A-I) The vessels were stained for LYVE-1 (red). Sprouts formed by adenoviral Ang1 or VEGF-C are indicated with arrowheads in panels A, B, and D. Fusion of individual sprouts 4 days after AdVEGF-C administration is shown with an open arrowhead in panel E. At the 14-day time point, a dense network of lymphatic vessels can be seen in both Ang1- and VEGF-C-treated ears (C,F). (G-I) Lymphatic vessels after AdLacZ transduction. (J-K) Staining of Ang1 (red) and LYVE-1 (green). Localization of Ang1 positive cells (blue arrowheads) and lymphatic sprouts in the ear transduced with AdAng1 (J). AdLacZ-transduced (K) and AdAng1-transduced goat IgG-stained controls (L) are shown for comparison. Scale bars: 100 μm.