Ang1 induces proliferation of lymphatic endothelial cells. (A-C) Double staining of VEGFR-3 (red) and the proliferation marker phosphohistone H3 (green) 14 days after adenovirus transduction. Double-positive cells (arrowheads) are identified from confocal stacks by X and Y projections (white arrows). (D-G) PCNA/LYVE-1 double staining of ears from mice treated with the indicated adenoviral vectors. PCNA-positive LEC nuclei have been marked with arrowheads in panels D and E. (H) Quantification of the proportion of PCNA plus LYVE-1 double-positive cells relative to AdLacZ-negative control samples. Bars represent mean values ± SEM (n ≤ 3). Scale bars: 100 μm.