Characterization of SDF-1α and SDF-1 peptide cleavage by purified CPN. (A) SDS-PAGE analysis of purified CPN. Lane 1: 1 μg and lane 2: 4 μg (stained with Coomassie). (B) SDF-1α cleavage by CPN evaluated by Western blotting with specific antibodies. Lane 1: SDF-1α was incubated for 10 minutes at room temperature with PBS alone; lane 2: purified CPN at 10 μg/mL; lane 3: 50 μg/mL. (C) SDF-1α cleavage by CPN evaluated by MALDI. SDF-1α was incubated for 10 minutes at room temperature in PBS or with PBS containing purified CPN at 10 μg/mL. (D) RP-HPLC tracing for SDF-1 peptide treated for 10 minutes with 0.1 μg purified CPN.