SHIP1 inhibits LPS-induced MAPKs activation in TLR4-reconstituted COS7 cells. COS7 cells were cotransfected with 1 μg TLR4, 1 μg MD2, and 1 μg CD14 plasmids (TLR/M/C) to establish TLR4-reconstituted cells. Together with TLR/M/C plasmids, the cells were also transfected with 2 μg pBK-CMV/150.8SHIP1 (SHIP1) or its control empty vector pBK-CMV (CMV) and cultured for 48 hours. The cells were stimulated with 100 ng/mL LPS for the indicated time periods. The phosphorylation of ERK1/2, p38, JNK, and degradation of IκB-α were detected by Western blotting. Similar results were observed in 3 separate experiments.