Hypoxia-reoxygenation increases expression of senescence-associated proteins. (A) Reoxygenated Fancc^–/– LTBMC cells showed strong immunostaining for p53 and p21^Waf1. The hematopoietic cells harvested from a 2-week LTBMC were stained with the antibodies against p53 and p21^Waf1 and then counterstained with DAPI. Representative photomicrographs of p53 and p21^Waf1 immunofluorescent staining are shown. (B) Expression of p53 and p21^Waf1 as analyzed by immunoblotting. Equal loading was ensured by reprobing the blot with antibody for β-actin. (C) Reoxygenated Fancc^–/– LTBMC cells increased moderately the accumulation of p16^Ink4a, but not p19^Arf. The hematopoietic cells harvested from a 2-week LTBMC were stained with the antibodies against p16^Ink4a and p19^Arf. (D) Expression of p16^Ink4a and p19^Arf in WT and Fancc^–/– LTBMC cells, as analyzed by RT-PCR.