LYVE-1 expression in LECs and liver sinusoids. Immunostaining with ALY7 in tissue sections (A-B). Cross-sections were prepared from intestines of adult mice and stained with ALY7 (red color in panels A-B), CD34 mAb (green color in panel A), and CD31/PECAM-1 mAb (green color in panel B). Note that all LYVE-1⁺ cells are also CD31⁺ but CD34^-. (C) Cross-sections were prepared from liver of E14 embryos and stained with ALY7. Note that ALY7 specifically detects lymphatic vessels (arrows) but not blood vessels (arrowheads). ALY7 also detects sinusoid blood vessels in liver (open arrowheads). Scale bars indicate 100 μm.