Tie2 expression in LYVE-1⁺LECs. (A-C) Cross-sections from intestines of adult mice were stained with Tie2 mAb (green color in panel A) and ALY7 (red color in panel B). Note the Tie2 immunoreactivity in BECs and the weaker staining seen in LECs (arrows). Double staining of LYVE-1 and Tie2 demonstrates that LYVE-1⁺ LECs are Tie2⁺ (merged staining in panel C). Scale bars indicate 100 μm.