Protein levels of iron-related genes in the duodenum of hepcidin-deficient mice. (A) Western blot analysis of DMT1, Dcytb, and ferroportin (on 80 μg crude membrane preparation) from the duodenum of Usf2^-/- and Usf2^+/- mice. Molecular weight markers are indicated on the left. A typical experiment, representative of at least 3 independent experiments, is shown. (B) Immunohistochemistry using antimouse DMT1, Dcytb, and ferroportin antibodies on the duodenum of Usf2^-/- and Usf2^+/- mice. Original magnification, ×10.