Figure 2.
Nuclear HIF-1α protein induction following treatment with CuCl2. Hep3B hepatoma (subline HRB5) (A) and HeLa cervical carcinoma (B) cell lines were treated with the CuCl2 concentrations indicated for 6 and 24 hours. HIF-1α protein was detected by immunoblotting using a monoclonal anti-HIF-1α antibody. Hypoxia (A) and the iron chelator CPX (B) were used as positive controls. (C) Hep3B hepatoma cells (subline HRB5) were exposed to normoxic (20% O2) or hypoxic (1% O2) conditions or to 125 μM CuCl2 for 24 hours. The cells were prepared for indirect immunofluorescence analysis as described in “Materials and methods.” Original magnification, × 630.
