Telomerase activity in transduced T lymphocytes. Telomerase activity in cultured clones (cl.) and polyclonal (p.c.) populations of CD4+ T cells transduced with hTERT or a GFP control vector. After transduction, cells were sorted for GFP expression and further expanded. Two thousand transduced cells were analyzed for telomerase activity using the TRAP assay. T lymphocytes transduced with hTERT exhibited high telomerase activity 10 days after stimulation. At this time point no telomerase activity was detected in GFP control cells. Negative control (n.c.) indicates no cell extract; positive control, extracts obtained from K562 cell line. Internal control shows PCR products amplified from an internal control primer to ensure equal loading of the samples.33 Results shown are representative of 3 separate experiments.
