Figure 4.
Figure 4. Survival of CLL B cells with unmutated and mutated VH genes after exposure to sol- and imm-IgM. (A) CLL B cells from patients with unmutated (n = 17, left panel) and mutated (n = 20, right panel) VH genes were cultured for 48 hours in the presence of sol-IgM (10 μg/mL complete antibody or 5 μg/mL F(ab′)2 fragments) or 10 μg/mL imm-IgM. Apoptosis was investigated by annexin-V FITC and PI double staining. Values represent the mean percentage of viable (double negative) cells; error bars indicate SEM. (B) Flow cytometry analysis of a representative case with unmutated VH genes (G83).

Survival of CLL B cells with unmutated and mutated VH genes after exposure to sol- and imm-IgM. (A) CLL B cells from patients with unmutated (n = 17, left panel) and mutated (n = 20, right panel) VH genes were cultured for 48 hours in the presence of sol-IgM (10 μg/mL complete antibody or 5 μg/mL F(ab′)2 fragments) or 10 μg/mL imm-IgM. Apoptosis was investigated by annexin-V FITC and PI double staining. Values represent the mean percentage of viable (double negative) cells; error bars indicate SEM. (B) Flow cytometry analysis of a representative case with unmutated VH genes (G83).

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