Delayed CD4⁺ T-cell homing to skin and small intestine of recipients of cells from CCR6-deficient donors. (A) Flow cytometric analysis of CD45⁺ and CD4⁺ cells infiltrated in ear skin. (B) Real-time quantitative RT-PCR analysis of CD4 mRNA levels in the small intestine of naive T-cell recipients. Data represent the mean ± SD (n = 2-4 mice/group/time-point) and illustrate the x-fold increase in mice that received transplants compared with mice that did not receive transplants. (A-B) ○ indicates WT donors; □, CCR6-deficient donors.