Figure 2.
![Figure 2. Erythroid differentiation is inhibited in human CD34+ cells expressing RPS19 shRNAs. In panel A, mean glycophorin A (Gly-A) expression, assayed by flow cytometry, is shown as a percentage of the luciferase (luc) shRNA control (mean and SEM). In panel B, mean CD11b expression is displayed as a percentage of the control. Methylcellulose colony assays were performed with cells expressing control or RPS19 shRNAs. The number of BFU-E colonies is presented in panel C, and the number of CFU-GM colonies is presented in panel D (mean [SEM]). *P < .001 by 2-tailed Student t test; **P < .05.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/12/10.1182_blood-2004-08-3313/5/zh80120579690002.jpeg?Expires=1735817013&Signature=eyb4NPaqq5eBd65vt0fC-UrpMJMu~adKGrPKNdjS~Hcyg8DgmREPvXMahPuwaqqsL3mru6qv3cfzCr1hisprZ0EN3uuCbp5ONE09b4b6rrCccYDlFaBjw2x8LYWzKBRLm9JHbqHo35-hSxC-njJfC1bTgxMCs1oEiSGglC3m4LOWWAy34a8xuV2sPHGAiWx8YokJHReXYQNj5u3g4SK8vW5CyjGkGdkgPfjdW3wrV1Ks~FROcg3~dgU4cLzynAhK6EzSxy-lnypRSMYSuNPtxmuxJsSQC7uTOnD3G8thdB5b5qddLgUuLd5XSj9dsJ-I1DQh~nqaDW6xPMB1RVL3gQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Erythroid differentiation is inhibited in human CD34+cells expressing RPS19 shRNAs. In panel A, mean glycophorin A (Gly-A) expression, assayed by flow cytometry, is shown as a percentage of the luciferase (luc) shRNA control (mean and SEM). In panel B, mean CD11b expression is displayed as a percentage of the control. Methylcellulose colony assays were performed with cells expressing control or RPS19 shRNAs. The number of BFU-E colonies is presented in panel C, and the number of CFU-GM colonies is presented in panel D (mean [SEM]). *P < .001 by 2-tailed Student t test; **P < .05.
