Modulation of Tc1 versus Tc2 cytokine secretion by A2A receptor agonism. Purified Tc1 and Tc2 cells (0.5 × 106 cells/mL) were restimulated on day 5 of culture with 3/28 beads in the absence of CGS (-; vehicle control) or with CGS (10-10 to 10-6 M). After 24 hours, supernatants were analyzed for cytokine content by ELISA. (A) Normalized cytokine secretion of CGS-treated Tc1 and Tc2 cultures. Effect of CGS is represented as percentage of control cytokine secretion (costimulation in the presence of vehicle); mean and SEM of 3 experiments are shown. (B) CGS inhibits low-level IL-2 secretion emanating from the Tc2 population. Absolute values for IL-2 and IFN-γ secretion from Tc2 cells exposed to CGS or vehicle control. (C) CGS inhibits TNF-α secretion from both Tc1 and Tc2 effectors. Absolute values for TNF-α secretion from Tc1 and Tc2 cells exposed to CGS or vehicle control; mean and SEM of 3 experiments are shown.
