Cytotoxic activity of HD-aAPC– and LD-aAPC–induced CD8⁺ CTLs of 5 donors. HD-aAPC– (top) and LD-aAPC– (bottom) induced CTLs were purified on day 35 (d35) after in vitro priming to more than 99% HA-1 tetramer–positive cells (unless indicated otherwise). *Assays with frozen and thawed aAPC-induced CTLs. Effector-target (E/T) ratios are given on the horizontal axis. Incubation time of assays was 3 hours (except where indicated).