Phenotypic characterization of CD8⁺ LD-aAPC–induced CTLs from D2 after MACS purification of tetramer-positive cells and 2 weeks of in vitro expansion. The dot plot shows staining with HA-1 tetramer PE and CD8 APC Ab. The percentage of CD8⁺ HA-1 tetramer–positive cells is given in the right upper quadrant. A tetramer loaded with the HIV peptide was used as a control (not shown). Histograms show expressed antigens (open curves) and the respective controls (shaded curves). CD62L indicates CD62 ligand; FasL, Fas ligand.