TCR repertoires of tetramer-sorted HD- and LD-aAPC–induced HA-1–specific CTLs. CDR3-size spectratyping was applied to analyze the TCR repertoire of HD-aAPC– and LD-aAPC––induced CTLs from D1 (left) and D2 (right). The upper panels show the respective spectratyping gels with the 24 Vβ families distributed on 14 lanes. The amplicons displayed on each lane are detailed in “Materials and methods.” Lower panels show histograms of selected Vβ families as representative examples of skewed TCR repertoires of HD- and LD-aAPC–induced CTLs. Although usage of Vβ families varied between HD- and LD-aAPC–induced CTLs in both donors, Vβ6 using CDR3s was detected consistently in HD- as well as in LD-aAPC–induced CTLs from both donors.