Transduction of the long-term reconstituting hematopoietic stem cell population (c-Kit⁺ Sca-1⁺ Lin^- Thy1.1^-) with scTCR-EGFP MFG retroviral vector. (A) Schematic diagram of the scTCR-EGFP MFG retroviral vector. (B) BM cells transduced with the scTCR-EGFP MFG retroviral vector were stained on day 7 in culture for hematopoietic stem cell surface markers (c-Kit and Sca-1) and for lineage markers (Lin). Cells that expressed high levels of Sca-1 and c-Kit (C) and that lacked expression of Lin (D), R2, and R3 were gated on and were plotted against EGFP (E). FSC indicates forward scatter. (F) Mock-transduced BM cells. All cells in culture were Thy1.1^- (not shown). Percentages are percentages of EGFP⁺ cells.