Figure 4.
Detection of scTCR-expressing cells at various times after reconstitution with transduced BM cells. Mice were bled at indicated time points, and leukocytes were stained for the appropriate cell surface markers plotted on the y-axis: (A) GR-1 for granulocytes, (B) Mac-3 or F4/80 for monocytes/macrophages, (C) DX5 for NK cells, (D) CD3 for T cells, and (E) B220 for B cells. EGFP expression is plotted on the x-axis. Percentages of EGFP-positive cells in each lineage are indicated.