GVHD is not effectively propagated through direct allorecognition. (A) Lethally irradiated (900 cGy) Balb mice received transplants of TCD B6 BM alone (□, n = 3) or together with 3 × 105 B6 spleen cells (∼ 1 × 105 T cells; ▪, n = 8). Mice were killed 18 to 24 days after bone marrow transplantation (BMT). (A) Serial weight curves are shown in panel A. Mean overall GVHD score and pathology score in the colon are shown in panel B. Results are representative of 5 independent experiments. Pooled spleen cell suspensions from mice that had received transplants as in panel A (containing either 0.4 or 1 × 106 T cells in 2 independent experiments) were then transplanted along with B6 Rag-1 BM (10 × 106 cells) into lethally irradiated Balb mice (n = 7). Data indicate percentage of body weight change (C) and mean overall and colon GVHD scores (D). Error bars depict SEM.
